primary rabbit anti- anoctamin- 1 (Alomone Labs)
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Primary Rabbit Anti Anoctamin 1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+primary+antibodies/Anti-TMEM16A+(ANO1)+(extracellular)+Antibody/pm42277581-77-29-38
Average 93 stars, based on 12 article reviews
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Blocking Assay:Article Title: Caveolar remodeling is a critical mechanotransduction mechanism of the stretch-induced L-type Ca 2+ channel activation in vascular myocytes. Article Snippet: Activation of L-type voltage-dependent Ca channels (VDCCL) by membrane stretch contributes to many biological responses such as myogenic contraction of arteries.. However, mechanism for the stretch-induced VDCCL activation is unclear.. In this study, we examined the hypothesis that caveolar remodeling and its related signaling cascade contribute to the stretch-induced activation of VDCCL in rat mesenteric arterial smooth muscle cells. Incubation:Article Title: Caveolar remodeling is a critical mechanotransduction mechanism of the stretch-induced L-type Ca 2+ channel activation in vascular myocytes. Article Snippet: Activation of L-type voltage-dependent Ca channels (VDCCL) by membrane stretch contributes to many biological responses such as myogenic contraction of arteries.. However, mechanism for the stretch-induced VDCCL activation is unclear.. In this study, we examined the hypothesis that caveolar remodeling and its related signaling cascade contribute to the stretch-induced activation of VDCCL in rat mesenteric arterial smooth muscle cells. Article Title: Intrathecal Resiniferatoxin Modulates TRPV1 in DRG Neurons and Reduces TNF-Induced Pain-Related Behavior Article Snippet: .. To investigate the relative protein level of TRPV1, slices were incubated with Article Title: Functional Coupling Between Voltage-Dependent Sodium Channels and Activation of the Ca2+ Signaling That Mediates Endothelial Cell Migration Article Snippet: Seven fields per coverslip were examined using a Nikon Eclipse E600 FN1 microscope (Nikon Corporation, Tokyo, Japan), and the results were expressed as the angiogenic index according to the following relation: (Total cells + connected cells)/total cells × (1-nonconnected cells), where Total cells is the number of total cells in the field, connected cells is the number of the cells that form tubular structures and non-connected cells is the number of the cells outside of tubular structures. .. Endothelial cells were fixed with 4% PFA, blocked with 3% BSA in PBS and incubated overnight at 4 ◦C with a mouse primary antibody directed against Cav-1 (1:100; Thermo Scientific, Rockford, IL, USA), Article Title: Impaired regulation of heart rate and sinoatrial node function by the parasympathetic nervous system in type 2 diabetic mice Article Snippet: Protein samples (20 μg/lane) were prepared in Laemmli sample buffer (Bio-Rad) containing 355 mM 2- marcaptoethanol (Bio-Rad) separated by 7.5% SDS-polyacrylamide gels (SDS-PAGE) and transferred onto BiotraceTM NT nitrocellulose Transfer Membrane (VWR). .. The membrane was blocked with 1% casein in tris-buffered saline (TBS; Bio-Rad) for 1 hour and incubated overnight at 40C with Membrane:Article Title: Impaired regulation of heart rate and sinoatrial node function by the parasympathetic nervous system in type 2 diabetic mice Article Snippet: Protein samples (20 μg/lane) were prepared in Laemmli sample buffer (Bio-Rad) containing 355 mM 2- marcaptoethanol (Bio-Rad) separated by 7.5% SDS-polyacrylamide gels (SDS-PAGE) and transferred onto BiotraceTM NT nitrocellulose Transfer Membrane (VWR). .. The membrane was blocked with 1% casein in tris-buffered saline (TBS; Bio-Rad) for 1 hour and incubated overnight at 40C with Saline:Article Title: Impaired regulation of heart rate and sinoatrial node function by the parasympathetic nervous system in type 2 diabetic mice Article Snippet: Protein samples (20 μg/lane) were prepared in Laemmli sample buffer (Bio-Rad) containing 355 mM 2- marcaptoethanol (Bio-Rad) separated by 7.5% SDS-polyacrylamide gels (SDS-PAGE) and transferred onto BiotraceTM NT nitrocellulose Transfer Membrane (VWR). .. The membrane was blocked with 1% casein in tris-buffered saline (TBS; Bio-Rad) for 1 hour and incubated overnight at 40C with Stripping Membranes:Article Title: mGluR3 Activation Recruits Cytoplasmic TWIK-1 Channels to Membrane that Enhances Ammonium Uptake in Hippocampal Astrocytes. Article Snippet: After secondary antibody incubation (Jackson ImmunoResearch Laboratories, Maine, USA), immunoreactivity was detected with an enhanced chemiluminescent detection (Thermo Fisher Scientific, Rockford, IL). .. After detecting TWIK-1 immunoreactivity, the membranes were stripped with stripping buffer (0.4 M glycine, 0.2 % SDS, and 2 % Tween 20, pH 2.0) and re-probed with the following |
