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primary rabbit anti- anoctamin- 1  (Alomone Labs)


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    Structured Review

    Alomone Labs primary rabbit anti- anoctamin- 1
    Primary Rabbit Anti Anoctamin 1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+primary+antibodies/Anti-TMEM16A+(ANO1)+(extracellular)+Antibody/pm42277581-77-29-38
    Average 93 stars, based on 12 article reviews
    primary rabbit anti- anoctamin- 1 - by Bioz Stars, 2026-09
    93/100 stars

    Images

    Related Articles

    Blocking Assay:

    Article Title: Caveolar remodeling is a critical mechanotransduction mechanism of the stretch-induced L-type Ca 2+ channel activation in vascular myocytes.
    Article Snippet: Activation of L-type voltage-dependent Ca channels (VDCCL) by membrane stretch contributes to many biological responses such as myogenic contraction of arteries.. However, mechanism for the stretch-induced VDCCL activation is unclear.. In this study, we examined the hypothesis that caveolar remodeling and its related signaling cascade contribute to the stretch-induced activation of VDCCL in rat mesenteric arterial smooth muscle cells.

    Incubation:

    Article Title: Caveolar remodeling is a critical mechanotransduction mechanism of the stretch-induced L-type Ca 2+ channel activation in vascular myocytes.
    Article Snippet: Activation of L-type voltage-dependent Ca channels (VDCCL) by membrane stretch contributes to many biological responses such as myogenic contraction of arteries.. However, mechanism for the stretch-induced VDCCL activation is unclear.. In this study, we examined the hypothesis that caveolar remodeling and its related signaling cascade contribute to the stretch-induced activation of VDCCL in rat mesenteric arterial smooth muscle cells.

    Article Title: Intrathecal Resiniferatoxin Modulates TRPV1 in DRG Neurons and Reduces TNF-Induced Pain-Related Behavior
    Article Snippet: .. To investigate the relative protein level of TRPV1, slices were incubated with rabbit primary antibodies specific for TRPV1 receptor protein (1 : 500; Alomone Labs, Israel). .. Mouse primary antibody for NeuN (1 : 500; Abcam) was used as a neuronal marker.

    Article Title: Functional Coupling Between Voltage-Dependent Sodium Channels and Activation of the Ca2+ Signaling That Mediates Endothelial Cell Migration
    Article Snippet: Seven fields per coverslip were examined using a Nikon Eclipse E600 FN1 microscope (Nikon Corporation, Tokyo, Japan), and the results were expressed as the angiogenic index according to the following relation: (Total cells + connected cells)/total cells × (1-nonconnected cells), where Total cells is the number of total cells in the field, connected cells is the number of the cells that form tubular structures and non-connected cells is the number of the cells outside of tubular structures. .. Endothelial cells were fixed with 4% PFA, blocked with 3% BSA in PBS and incubated overnight at 4 ◦C with a mouse primary antibody directed against Cav-1 (1:100; Thermo Scientific, Rockford, IL, USA), rabbit primary antibodies directed against Nav1.2 (1:350; Alomone Laboratories, Jerusalem, Israel) or Nav1.6 (1:350; Alomone Laboratories, Jerusalem, Israel), and then with Alexa 488-labeled goat anti-mouse or Alexa 568-labeled goat anti-rabbit secondary antibodies (1:2000; Molecular Probes, Eugene, OR, USA) for 1 h at room temperature, as appropriate. .. The fluorescence signal was examined using a Nikon spectral C2si confocal microscope (Nikon Instruments, Melville, NY, USA).

    Article Title: Impaired regulation of heart rate and sinoatrial node function by the parasympathetic nervous system in type 2 diabetic mice
    Article Snippet: Protein samples (20 μg/lane) were prepared in Laemmli sample buffer (Bio-Rad) containing 355 mM 2- marcaptoethanol (Bio-Rad) separated by 7.5% SDS-polyacrylamide gels (SDS-PAGE) and transferred onto BiotraceTM NT nitrocellulose Transfer Membrane (VWR). .. The membrane was blocked with 1% casein in tris-buffered saline (TBS; Bio-Rad) for 1 hour and incubated overnight at 40C with rabbit primary antibodies separately including HCN1 1:500 (Alomone Labs), HCN2 1:500 (Alomone Labs), HCN4 1:500 (Alomone Labs), M2R 1:500 (Abcam), Kir3.1 1:1000 (Abcam), Kir3.4 1:1000 (Abcam) and RGS4 1:500 (Aviva Systems Biology). .. The membrane was washed 3 times with TBST (TBS with 1% Tween 20 (Bio-Rad)) and incubated with StarBright700 anti-Rabbit secondary antibody (Bio-Rad) at 1:10000 together with Rhodamine anti-GAPDH at 1:5000 (Bio-Rad) for 1 hour at room temperature.

    Membrane:

    Article Title: Impaired regulation of heart rate and sinoatrial node function by the parasympathetic nervous system in type 2 diabetic mice
    Article Snippet: Protein samples (20 μg/lane) were prepared in Laemmli sample buffer (Bio-Rad) containing 355 mM 2- marcaptoethanol (Bio-Rad) separated by 7.5% SDS-polyacrylamide gels (SDS-PAGE) and transferred onto BiotraceTM NT nitrocellulose Transfer Membrane (VWR). .. The membrane was blocked with 1% casein in tris-buffered saline (TBS; Bio-Rad) for 1 hour and incubated overnight at 40C with rabbit primary antibodies separately including HCN1 1:500 (Alomone Labs), HCN2 1:500 (Alomone Labs), HCN4 1:500 (Alomone Labs), M2R 1:500 (Abcam), Kir3.1 1:1000 (Abcam), Kir3.4 1:1000 (Abcam) and RGS4 1:500 (Aviva Systems Biology). .. The membrane was washed 3 times with TBST (TBS with 1% Tween 20 (Bio-Rad)) and incubated with StarBright700 anti-Rabbit secondary antibody (Bio-Rad) at 1:10000 together with Rhodamine anti-GAPDH at 1:5000 (Bio-Rad) for 1 hour at room temperature.

    Saline:

    Article Title: Impaired regulation of heart rate and sinoatrial node function by the parasympathetic nervous system in type 2 diabetic mice
    Article Snippet: Protein samples (20 μg/lane) were prepared in Laemmli sample buffer (Bio-Rad) containing 355 mM 2- marcaptoethanol (Bio-Rad) separated by 7.5% SDS-polyacrylamide gels (SDS-PAGE) and transferred onto BiotraceTM NT nitrocellulose Transfer Membrane (VWR). .. The membrane was blocked with 1% casein in tris-buffered saline (TBS; Bio-Rad) for 1 hour and incubated overnight at 40C with rabbit primary antibodies separately including HCN1 1:500 (Alomone Labs), HCN2 1:500 (Alomone Labs), HCN4 1:500 (Alomone Labs), M2R 1:500 (Abcam), Kir3.1 1:1000 (Abcam), Kir3.4 1:1000 (Abcam) and RGS4 1:500 (Aviva Systems Biology). .. The membrane was washed 3 times with TBST (TBS with 1% Tween 20 (Bio-Rad)) and incubated with StarBright700 anti-Rabbit secondary antibody (Bio-Rad) at 1:10000 together with Rhodamine anti-GAPDH at 1:5000 (Bio-Rad) for 1 hour at room temperature.

    Stripping Membranes:

    Article Title: mGluR3 Activation Recruits Cytoplasmic TWIK-1 Channels to Membrane that Enhances Ammonium Uptake in Hippocampal Astrocytes.
    Article Snippet: After secondary antibody incubation (Jackson ImmunoResearch Laboratories, Maine, USA), immunoreactivity was detected with an enhanced chemiluminescent detection (Thermo Fisher Scientific, Rockford, IL). .. After detecting TWIK-1 immunoreactivity, the membranes were stripped with stripping buffer (0.4 M glycine, 0.2 % SDS, and 2 % Tween 20, pH 2.0) and re-probed with the following rabbit primary antibodies sequentially: anti-Kir4.1 (1:600, Alomone Labs, Jerusalem, Israel), anti-Na+/K+ ATPase α2 (+) polypeptide (ATP1α2) (1:1000; Abgent, San Diego, CA), anti-GAPDH (1:8000, Sigma-Aldrich, St . Louis, MO), and anti-GFAP (1:500, DAKO, Carpinteria, CA). ..



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    Image Search Results


    Composite hydrogel promotes the polarization of BV2 cells to M2 types and alleviates PC12 cell pyroptosis in vitro inflammatory environment. (A) Representative western blots showing protein expression of iNOS and Arg-1 in each group, β-actin was utilized as a loading control. (B) Quantitative analysis of relative expression of iNOS and Arg-1. (C) Representative immunofluorescence images of CD68 positive and iNOS positive BV2 cells (scale bar: 20 μm). (D) Representative immunofluorescence images of CD68 positive and Arg-1 positive BV2 cells (scale bar: 20 μm). (E, F) Quantitative analysis of relative fluorescence intensity of iNOS and Arg-1. (G) Representative western blots showing the expression of NLRP3, Caspase-1, IL-1β, ASC, GSDMD-N, and IL-18 protein associated with pyroptosis, β-actin was utilized as a loading control. (H) PI staining of PC12 cells in each group (scale bar, 100 μm). (I) Quantitative analysis of PI staining of PC12 cells (n = 3). (J) Quantitative analysis of relative expression of NLRP3, Caspase-1, IL-1β, ASC, GSDMD-N and IL-18 (n = 3). The data are presented as the means ± SEMs (n = 3); ∗p < 0.05, indicates significant differences; ns, is not significant. Statistical analysis was performed using two-way ANOVA followed by Tukey's multiple comparison test.

    Journal: Bioactive Materials

    Article Title: 3D-MSCs apoptotic bodies-integrated conductive hydrogel mitigates spinal cord injury via immunoregulation and alleviating neuronal pyroptosis

    doi: 10.1016/j.bioactmat.2026.01.043

    Figure Lengend Snippet: Composite hydrogel promotes the polarization of BV2 cells to M2 types and alleviates PC12 cell pyroptosis in vitro inflammatory environment. (A) Representative western blots showing protein expression of iNOS and Arg-1 in each group, β-actin was utilized as a loading control. (B) Quantitative analysis of relative expression of iNOS and Arg-1. (C) Representative immunofluorescence images of CD68 positive and iNOS positive BV2 cells (scale bar: 20 μm). (D) Representative immunofluorescence images of CD68 positive and Arg-1 positive BV2 cells (scale bar: 20 μm). (E, F) Quantitative analysis of relative fluorescence intensity of iNOS and Arg-1. (G) Representative western blots showing the expression of NLRP3, Caspase-1, IL-1β, ASC, GSDMD-N, and IL-18 protein associated with pyroptosis, β-actin was utilized as a loading control. (H) PI staining of PC12 cells in each group (scale bar, 100 μm). (I) Quantitative analysis of PI staining of PC12 cells (n = 3). (J) Quantitative analysis of relative expression of NLRP3, Caspase-1, IL-1β, ASC, GSDMD-N and IL-18 (n = 3). The data are presented as the means ± SEMs (n = 3); ∗p < 0.05, indicates significant differences; ns, is not significant. Statistical analysis was performed using two-way ANOVA followed by Tukey's multiple comparison test.

    Article Snippet: Primary antibodies against NLRP3 (cat. no. 15101) were obtained from Cell Signaling Technology (Beverly, Massachusetts, USA).

    Techniques: In Vitro, Western Blot, Expressing, Control, Immunofluorescence, Fluorescence, Staining, Comparison

    The composite hydrogel inhibits post-SCI pyroptosis in neurons. (A) Immunofluorescence images of residual neurons existing in the anterior horn of the spinal cord (scale bar, 500 μm and 200 μm). (B) Immunofluorescence image of Caspase-1 expression of neurons 3 days after SCI (scale bar, 20 μm). (C) Quantitative analysis of relative fluorescence intensity of Caspase-1 protein expression in neurons within the specified groups (n = 3). (D) Immunofluorescence image of GSDMD-N expression of neurons 3 days after SCI (scale bar, 20 μm). (E) Quantitative analysis of relative fluorescence intensity of GSDMD-N protein expression in neurons within the specified groups (n = 3). (F) Representative western blots showing the expression of NLRP3, Caspase-1, IL-1β, ASC, GSDMD-N, and IL-18 protein 3 days after SCI, GAPDH was utilized as a loading control. (G) Quantitative analysis of relative expression of NLRP3, Caspase-1, IL-1β, ASC, GSDMD-N and IL-18 (n = 3). The data are presented as the means ± SEMs (n = 3); ∗p < 0.05, indicates significant differences; ns, is not significant. Statistical analysis was performed using two-way ANOVA followed by Tukey's multiple comparison test.

    Journal: Bioactive Materials

    Article Title: 3D-MSCs apoptotic bodies-integrated conductive hydrogel mitigates spinal cord injury via immunoregulation and alleviating neuronal pyroptosis

    doi: 10.1016/j.bioactmat.2026.01.043

    Figure Lengend Snippet: The composite hydrogel inhibits post-SCI pyroptosis in neurons. (A) Immunofluorescence images of residual neurons existing in the anterior horn of the spinal cord (scale bar, 500 μm and 200 μm). (B) Immunofluorescence image of Caspase-1 expression of neurons 3 days after SCI (scale bar, 20 μm). (C) Quantitative analysis of relative fluorescence intensity of Caspase-1 protein expression in neurons within the specified groups (n = 3). (D) Immunofluorescence image of GSDMD-N expression of neurons 3 days after SCI (scale bar, 20 μm). (E) Quantitative analysis of relative fluorescence intensity of GSDMD-N protein expression in neurons within the specified groups (n = 3). (F) Representative western blots showing the expression of NLRP3, Caspase-1, IL-1β, ASC, GSDMD-N, and IL-18 protein 3 days after SCI, GAPDH was utilized as a loading control. (G) Quantitative analysis of relative expression of NLRP3, Caspase-1, IL-1β, ASC, GSDMD-N and IL-18 (n = 3). The data are presented as the means ± SEMs (n = 3); ∗p < 0.05, indicates significant differences; ns, is not significant. Statistical analysis was performed using two-way ANOVA followed by Tukey's multiple comparison test.

    Article Snippet: Primary antibodies against NLRP3 (cat. no. 15101) were obtained from Cell Signaling Technology (Beverly, Massachusetts, USA).

    Techniques: Immunofluorescence, Expressing, Fluorescence, Western Blot, Control, Comparison